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Estradiol (E2)

Estradiol (E2)

Test ID: A858

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Estradiol (E2) is a hormone naturally produced in both genders, with much higher levels in females. Estradiol is essential for female reproductive health, and also plays important functions in the male reproductive system, skeletal system, skin health, nervous system, and cardiovascular system. Abnormal estradiol levels can affect fertility, sex drive, muscle and bone mass, growth, and skin health.



How to order a test


  • E2
  • Oestradiol

What is Included?

Measurement of estradiol levels by chemiluminescent microparticle immunoassay.

Turnaround Time

1 – 3 business days
The turnaround time is not guaranteed. The average turnaround time is 1 – 3 business days from the date that the sample arrives at the laboratory. Shipping time for the sample is not included. Additional time is required if the case requires confirmatory or reflex testing, or if the sample is insufficient, or if a recollection is required.

Related Documents

Special Instructions

Fertility testing in females: Samples should be collected on the third day of the menstrual cycle (third day of menstruation) for optimum results.


Preparation Before Specimen Collection


Specimen Type



50 μL in a microtainer


Microtainer (regular blood tube)

Collection Method

This test requires a blood sample from a finger prick. All supplies for sample collection are provided in the kit.

  1. First wash and dry hands. Warm hands aid in blood collection.
  2. Clean the finger prick site with the alcohol swab and allow to air dry.
  3. Use the provided lancet to puncture the skin in one quick, continuous and deliberate stroke.
  4. Wipe away the first drop of blood.
  5. Massage hand and finger to increase blood flow to the puncture site. Angle arm and hand downwards to facilitate blood collection on the fingertip.
  6. Drip blood into the microtainer tube.
  7. Dispose of all sharps safely and return sample to the laboratory in the provided prepaid return shipping envelope.

NOTES: Avoid squeezing or ‘milking’ the finger excessively. If more blood is required and blood flow stops, perform a second skin puncture on another finger. Do not touch the fingertip.

Specimen Storage

Maintain specimen at temperatures between 2°C and 30°C during storage and transport.

Specimen Stability

Blood samples can be refrigerated or kept at room temperature for up to 7 days.

Causes for Rejection

  • Incorrect or incomplete patient identification
  • Incorrect specimen collection
  • Inappropriate storage and transport conditions
  • Incorrect specimen volume



To measure estradiol levels in a blood sample to aid in the investigation of fertility and hormonal changes around menopause, for the support of in vitro fertilization, and to aid in the investigation of hormone levels in males.


  • This report is not intended for use in medico-legal applications.
  • These results should be interpreted in conjunction with other laboratory and clinical information.
  • Correct specimen collection and handling is required for optimal assay performance.
  • Assay interference may occur in specimens from individuals routinely exposed to animals or to animal serum products. Additional clinical or diagnostic information may be required for these specimens.
  • Estradiol results obtained from different assay methods cannot be used interchangeably in serial testing.
  • Estradiol levels fluctuate throughout each menstrual cycle in reproductive age females, so the timing of sample collection may influence the interpretation of the estradiol level.


Chemiluminescent microparticle immunoassay (Alinity i Estradiol assay)

Reference Intervals

Normal menstruating females
– Follicular phase: 20 – 350 pg/mL
– Mid-cycle: 150 – 750 pg/mL
– Luteal phase: 30 – 450 pg/mL

Postmenopausal females: ≤ 20 pg/mL
Males: 10 – 50 pg/mL

These reference ranges were obtained from Pagana KD, Pagana TJ, Pagana TN. Mosby’s Diagnostic & Laboratory Test Reference. 15th ed. St. Louis, Mo: Elsevier; 2021.