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Sex Hormone Binding Globulin (SHBG)

Sex Hormone Binding Globulin (SHBG)

Test ID: A908

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Sex hormone binding globulin (SHBG) is a transport protein for testosterone. It is synthesized in the liver, with synthesis and secretion controlled by estrogen. Testosterone bound to SHBG is in a biologically inactive form; hence SHBG levels regulate testosterone activity in the blood.

Elevated SHBG levels often occur in patients with hyperthyroidism and liver cirrhosis, as well as during pregnancy or when oral contraceptives or antiepileptic drugs are taken. Decreased SHBG concentrations can occur in hypothyroidism, polycystic ovarian syndrome (PCOS), obesity, hirsutism, elevated androgen levels, alopecia, and acromegaly.



How to order a test


  • Testosterone-binding globulin

What is Included?

Measurement of SHBG levels in a blood sample by chemiluminescent microparticle immunoassay.

Turnaround Time

1 – 3 business days
The turnaround time is not guaranteed. The average turnaround time is 1 – 3 business days from the date that the sample arrives at the laboratory. Shipping time for the sample is not included. Additional time is required if the case requires confirmatory or reflex testing, or if the sample is insufficient, or if a recollection is required.

Related Documents


Preparation Before Specimen Collection


Specimen Type



50 μL in a microtainer


Microtainer (regular blood tube)

Collection Method

This test requires a blood sample from a finger prick. All supplies for sample collection are provided in the kit.

  1. First wash and dry hands. Warm hands aid in blood collection.
  2. Clean the finger prick site with the alcohol swab and allow to air dry.
  3. Use the provided lancet to puncture the skin in one quick, continuous and deliberate stroke.
  4. Wipe away the first drop of blood.
  5. Massage hand and finger to increase blood flow to the puncture site. Angle arm and hand downwards to facilitate blood collection on the fingertip.
  6. Drip blood into the microtainer tube.
  7. Dispose of all sharps safely and return sample to the laboratory in the provided prepaid return shipping envelope.

NOTES: Avoid squeezing or ‘milking’ the finger excessively. If more blood is required and blood flow stops, perform a second skin puncture on another finger. Do not touch the fingertip.

Specimen Storage

Maintain specimen at temperatures between 2°C and 30°C during storage and transport.

Specimen Stability

Blood samples can be refrigerated or kept at room temperature for up to 7 days.

Causes for Rejection

  • Incorrect or incomplete patient identification
  • Incorrect specimen collection
  • Inappropriate storage and transport conditions
  • Incorrect specimen volume



To measure SHBG levels in a blood sample for the diagnosis of abnormal SHBG levels, which can affect testosterone activity.


  • This report is not intended for use in medico-legal applications. 
  • These results should be interpreted in conjunction with other laboratory and clinical information.
  • Correct specimen collection and handling is required for optimal assay performance. 
  • Interferences from medication or endogenous substances may affect results.


Chemiluminescent microparticle immunoassay (Alinity i SHBG Assay)

Reference Intervals

Adult Males: 11.2 – 78.1 nmol/L
Adult Females: 11.7 – 137.2 nmol/L

These reference ranges were obtained from the Alinity i SHBG package insert.